Quantification of CA 125 in peritoneal fluid: inhibition of the CA 125 response in the one-step immunoradiometric assay and establishment of a two-step assay format.
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منابع مشابه
Serum and Peritoneal Fluid CA 125 Levels Infertile Women with Pelvic Endometriosis in Urummia
SUMMARY Infertility has a special importance in gynecology in causing social, psychological and somatic prohlems. Endometriosis is a benign but progressive disorder in reproductive life and is more common in infertile women. CA 125 is a cell membrane glycoprotein. The endometrium is one the known source of this antigen and seems to be elevated in the pelvic endometriosis. In this study, from...
متن کاملEpitopes on CA 125 from cervical mucus and ascites fluid and characterization of six new antibodies. Third report from the ISOBM TD-1 workshop.
CA 125 is found in body fluids in a variety of molecular weight forms. The largest species are found in normal abdominal fluid and cervical mucus. The present study therefore incorporated CA 125 derived from these sources as well as ascites fluid to investigate if the source of CA 125 influenced epitope characterization. Ascites-derived CA 125 varied in size from about 190 to about 2,700 kD. Ce...
متن کاملMonoclonal Antibody Immunoradiometric Assay for an Antigenic Determinant (CA 125) Associated with Human Epithelial Ovarian Carcinomas1
CA 125 is an antigenic determinant expressed by greater than 80% of nonmucinous epithelial ovarian carcinomas. An immunoradiometric assay has been developed using a murine mono clonal antibody (OC125) to quantitate CA 125 in human serum. This immunoradiometric assay was optimized for specificity, sensitivity, and performance characteristics. Using a simultane ous immunoradiometric assay, the me...
متن کاملMonoclonal antibody immunoradiometric assay for an antigenic determinant (CA 125) associated with human epithelial ovarian carcinomas.
CA 125 is an antigenic determinant expressed by greater than 80% of nonmucinous epithelial ovarian carcinomas. An immunoradiometric assay has been developed using a murine monoclonal antibody (OC125) to quantitate CA 125 in human serum. This immunoradiometric assay was optimized for specificity, sensitivity, and performance characteristics. Using a simultaneous immunoradiometric assay, the mean...
متن کاملConfirmation of a false-positive result in CA 125 immunoradiometric assay caused by human anti-idiotypic immunoglobulin.
An immunoradiometric assay (IRMA) involving a monoclonal antibody (MAb OC125) to an ovarian carcinoma-associated antigenic determinant (CA 125) has been tested as one component in a strategy for early detection of epithelial ovarian cancer. We characterized one confirmed "false-positive" sample by murine antibody blocking studies, Western blotting, immunoaffinity, size-exclusion chromatography,...
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ورودعنوان ژورنال:
- Clinical chemistry
دوره 37 5 شماره
صفحات -
تاریخ انتشار 1991